【文獻標題】Fluoxastrobin-induced effects on acute toxicity, development toxicity,oxidative stress, ａnd DNA damage in Danio rerio embryos

【作者】Cheng Zhang，Jingwen Zhang，Lusheng Zhu，et.al

【作者單位】山東農(nóng)業(yè)大學（Shandong Agricultural University）

【文獻中引用產(chǎn)品】

斑馬魚8羥基脫氧鳥苷（8-OHdG）elisa試劑盒

【關(guān)鍵詞】96 h LC50，Delayed hatching，Teratogenicity，8-OHdG，AO-EB staining，Apoptosis

【DOI】https://doi.org/10.1016/j.scitotenv.2020.137069

【影響因子(IF)】5.92

【出版期刊】《Science of the Total Environment》

【產(chǎn)品原文引用】

2.7. Measurement of DNA damage induced by FLUO

To evaluate DNA damage to Danio rerio larvae induced by FLUO, 8-OHdG contents ａnd apoptosis were monitored. The supernatant was prepared from the homogenates prepared in Section 2.5 after centrifugation at 12,857g ａnd 4 °C for 10 min. The esultant solution was kept on ice during the entire procedure. A prior study (Guo et al., 2014)noted the significance of 8-OHdG in evaluating DNA damage. The 8-OHdH contents were monitored using an 8-OHdG ELISA kit (Hengyuan Biological Technology Co., Ltd., Shanghai, China) at 450 nm for 10 min (Multiskan MK3, Thermo Fisher Scientific, Massachusetts, USA) ａnd in units of ng/g protein. The total protein contents were assessed as in Section 2.6.As per Sun et al. (2019) ａnd Wang et al. (2018), apoptosis was monitored using acridine orange (AO)-ethidium bromide (EB) double dyeing kit (Solarbio Science & Technology Co., Beijing, China). At 96 hpf, ten larvae from each trial in Section 2.5 were rinsed twice with PBS (pH =7.4) ａnd dyed with 20 μL of AO ａnd EB working fluid (1/1, v/v) for 5 min ａnd then rinsed twice with PBS. Tritricaine (MS-222) was used for 3 min to anesthetize larvae for better observation. Then, the apoptosis of larvae was observed ａnd photographed under an inverted fluorescence microscope (xioVert. A1, Carl Zeiss AG, Oberkochen, Germany).

完整版PDF文獻請咨詢在線客服或者電話聯(lián)系我司業(yè)務員！

更多公司福利請關(guān)注“恒遠生物”微信公眾號！

原創(chuàng)作者：上海恒遠生物科技有限公司